The Doctor Who and Torchwood star was taken to hospital from the SECC Clyde Auditorium as a precaution. His injuries were not serious.
Barrowman had fallen off a live horse during the matinee show, which also stars the Krankies.
Janette Tough, "Wee Jimmy" Krankie, was almost killed in an on-stage accident in the city in 2004.
She suffered a fractured skull after falling from a mechanical beanstalk at the Pavilion Theatre.
An SECC spokeswoman said: "During today's matinee performance of Jack and the Beanstalk, John Barrowman suffered an injury on stage and has been taken to hospital as a precaution.
"His understudy, Greg Barrowman, has already stepped in to finish the rest of the show."
More Info and Video @ BBC